SLC22A12 Protein (AA 1-553) (Strep Tag)
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- Antigène Voir toutes SLC22A12 Protéines
- SLC22A12 (Solute Carrier Family 22 (Organic Anion/urate Transporter), Member 12 (SLC22A12))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-553
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette SLC22A12 protéine est marqué à la Strep Tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- Séquence
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MAFPELLDRV GGLGRFQLFQ TVALVTPILW VTTQNMLENF SAAVPHHRCW VPLLDNSTSQ ASIPGDLGPD VLLAVSIPPG PDQQPHQCLR FRQPQWQLTE SNATATNWSD AATEPCEDGW VYDHSTFRST IVTTWDLVCN SQALRPMAQS IFLAGILVGA AVCGHASDRF GRRRVLTWSY LLVSVSGTAA AFMPTFPLYC LFRFLLASAV AGVMMNTASL LMEWTSAQGS PLVMTLNALG FSFGQVLTGS VAYGVRSWRM LQLAVSAPFF LFFVYSWWLP ESARWLITVG KLDQGLQELQ RVAAVNRRKA EGDTLTMEVL RSAMEEEPSR DKAGASLGTL LHTPGLRHRT IISMLCWFAF GFTFYGLALD LQALGSNIFL LQALIGIVDF PVKTGSLLLI SRLGRRFCQV SFLVLPGLCI LSNILVPHGM GVLRSALAVL GLGCLGGAFT CITIFSSELF PTVIRMTAVG LCQVAARGGA MLGPLVRLLG VYGSWMPLLV YGVVPVLSGL AALLLPETKN LPLPDTIQDI QKQSVKKVTH DTPDGSILMS TRL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product SLC22A12 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- SLC22A12 (Solute Carrier Family 22 (Organic Anion/urate Transporter), Member 12 (SLC22A12))
- Autre désignation
- Slc22a12 (SLC22A12 Produits)
- Synonymes
- AI987855 Protein, OAT4L Protein, Rst Protein, Slc22al2 Protein, URAT1 Protein, RST Protein, solute carrier family 22 (organic anion/cation transporter), member 12 Protein, solute carrier family 22 member 12 Protein, Slc22a12 Protein, SLC22A12 Protein
- Sujet
- Solute carrier family 22 member 12 (Renal-specific transporter) (RST) (Urate anion exchanger 1) (URAT1) (Urate:anion antiporter SLC22A12),FUNCTION: Electroneutral antiporter that translocates urate across the apical membrane of proximal tubular cells in exchange for monovalent organic or inorganic anions (PubMed:14747372). Involved in renal reabsorption of urate and helps maintaining blood levels of uric acid (PubMed:14747372). Mediates urate uptake by an exchange with organic anions such as (S)-lactate and nicotinate, and inorganic anion Cl(-) (PubMed:14747372). Other inorganic anions such as Br(-), I(-) and NO3(-) may also act as counteranions that exchange for urate (By similarity). Also mediates orotate tubular uptake coupled with nicotinate efflux and to a lesser extent with lactate efflux, therefore displaying a potential role in orotate renal reabsorption. Orotate transport is Cl(-)-dependent (By similarity). {ECO:0000250|UniProtKB:Q96S37, ECO:0000269|PubMed:14747372}.
- Poids moléculaire
- 60.2 kDa
- UniProt
- Q8CFZ5
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