ARL6IP5 Protein (AA 1-188) (Strep Tag)
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- Antigène Voir toutes ARL6IP5 (Arl6ip5) Protéines
- ARL6IP5 (Arl6ip5) (ADP-Ribosylation-Like Factor 6 Interacting Protein 5 (Arl6ip5))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-188
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette ARL6IP5 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MDVNLAPLRA WDDFFPGSDR FARPDFRDIS KWNNRVVSNL LYYQTNYLVV AAMMISVVGF LSPFNMILGG VIVVLVFMGF VWAAHNKDIL RRMKKQYPTA FVMVVMLASY FLISMFGGVM VFVFGITLPL LLMFIHASLR LRNLKNKLEN KMEGIGLKKT PMGIILDALE QQEDNINKFA DYISKARE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product Arl6ip5 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- ARL6IP5 (Arl6ip5) (ADP-Ribosylation-Like Factor 6 Interacting Protein 5 (Arl6ip5))
- Autre désignation
- Arl6ip5 (Arl6ip5 Produits)
- Synonymes
- zp22 Protein, addicsin Protein, zgc:92811 Protein, wu:fj55a04 Protein, arl6ip5 Protein, cb937 Protein, zgc:101611 Protein, jmx Protein, jwa Protein, hp22 Protein, praf3 Protein, derp11 Protein, hspc127 Protein, gtrap3-18 Protein, 5930404D22Rik Protein, AV001879 Protein, Aip-5 Protein, Gtrap3-18 Protein, addiscin Protein, DERP11 Protein, GTRAP3-18 Protein, JWA Protein, PRAF3 Protein, ADP-ribosylation factor-like 6 interacting protein 5b Protein, ADP-ribosylation factor-like 6 interacting protein 5a Protein, ADP ribosylation factor like GTPase 6 interacting protein 5 Protein, ADP-ribosylation factor-like 6 interacting protein 5 Protein, ADP-ribosylation factor like GTPase 6 interacting protein 5 Protein, arl6ip5b Protein, arl6ip5a Protein, arl6ip5 Protein, Arl6ip5 Protein, ARL6IP5 Protein
- Sujet
- PRA1 family protein 3 (ADP-ribosylation factor-like protein 6-interacting protein 5) (ARL-6-interacting protein 5) (Aip-5) (Addicsin) (GTRAP3-18) (Glutamate transporter EAAC1-interacting protein) (Prenylated Rab acceptor protein 2) (Protein JWa),FUNCTION: Regulates intracellular concentrations of taurine and glutamate (By similarity). Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate in a PKC activity-dependent manner (PubMed:12119102, PubMed:18684713). Plays a role in the retention of SLC1A1/EAAC1 in the endoplasmic reticulum (By similarity). {ECO:0000250|UniProtKB:Q9ES40, ECO:0000269|PubMed:12119102, ECO:0000269|PubMed:18684713}.
- Poids moléculaire
- 21.6 kDa
- UniProt
- Q8R5J9
- Pathways
- Dicarboxylic Acid Transport
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