ENTPD4 Protein (AA 1-613) (Strep Tag)
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- Antigène Voir toutes ENTPD4 Protéines
- ENTPD4 (Ectonucleoside Triphosphate diphosphohydrolase 4 (ENTPD4))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-613
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette ENTPD4 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MGRIGISCLF PASWHFSISP VGCPRILNTN LRQIVVISIL AAAVSLLYFS VVIIRSKYGW LSKDKKFQRY LARVTDVEAT DTNNPSVNYG IVVDCGSSGS RIFVYCWPRH NGNPHDLLDI RQMRDKNRKP VVMKIKPGIS EFATSPEKVS DYISPLLSFA AEHVPRAKHK ETPLYILCTA GMRVLPESQQ KAILEDLLTD IPVHYDFLFS DSHAEVISGK QEGVYAWIGI NFVLGRFEHI EEDDEAVVEV NIPGSESSEA IVRKRTAGVL DMGGVSTQIA YEVPQTVSFA SSQQEEVAKN LLAEFNLGCD VHQTEHVYRV YVATFLGFGG NAARQRYEDR LFASTVQKNR LLGKQTGLTP DAPLLDPCLP LDIKDEIQQN GQTLYLQGTG DFDLCRETLQ PFMNKTNETQ TSLNGVYQPP IHFQNSEFYG FSEFYYCTED VLRMGGDYNA ARFTQAAKDY CATKWSILRE RFDRGLYASH ADLHRLKYQC FKSAWMFEVF HKGFSFPVTY KNLKTALQVY DKEVQWTLGA ILYRTRFLPL RDIRQEVFRA GHAHWRGVSF VYNHYLFSGC FLVVLLSILL YLLRLRRIHR RAPRTGSLWM EEGLPSQKGP GPL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product ENTPD4 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- ENTPD4 (Ectonucleoside Triphosphate diphosphohydrolase 4 (ENTPD4))
- Autre désignation
- Entpd4 (ENTPD4 Produits)
- Synonymes
- zgc:92668 Protein, wu:fd44h12 Protein, wu:fi73e02 Protein, LALP70 Protein, MGC142312 Protein, LAP70 Protein, LYSAL1 Protein, NTPDase-4 Protein, UDPase Protein, 1200014F22Rik Protein, 4930513O14Rik Protein, A430102M06Rik Protein, AI414893 Protein, Lysal1 Protein, mKIAA0392 Protein, ectonucleoside triphosphate diphosphohydrolase 4 Protein, ectonucleoside triphosphate diphosphohydrolase 4 L homeolog Protein, ENTPD4 Protein, entpd4 Protein, entpd4.L Protein, Entpd4 Protein
- Sujet
- Ectonucleoside triphosphate diphosphohydrolase 4 (NTPDase 4) (EC 3.6.1.15) (EC 3.6.1.6) (Lysosomal apyrase-like protein of 70 kDa) (Uridine-diphosphatase) (UDPase) (EC 3.6.1.42),FUNCTION: [Isoform 1]: Catalyzes the hydrolysis of nucleoside triphosphates and diphosphates in a calcium- or magnesium-dependent manner, with a preference for pyrimidines. Preferentially hydrolyzes UTP and TTP on UTP and TTP. AMP, ADP, ATP and UMP are not substrates. Preferentially activated by Ca(2+) over Mg(2+). {ECO:0000250|UniProtKB:Q9Y227}., FUNCTION: [Isoform 2]: Has a broad substrate specificity with the ability of cleaving all nucleotide di- and triphosphates with the exception of adenosine di- and triphosphate (ADP and ATP). Preferentially hydrolyzes CTP, UDP, CDP, GTP and GDP. Can use either Ca(2+) or Mg(2+) equally. {ECO:0000250|UniProtKB:Q9Y227}.
- Poids moléculaire
- 69.7 kDa
- UniProt
- Q9DBT4
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