Recombinant Human alpha-N-Acetylneuraminide alpha-2,8-Sialyltransferase/ST8SIA1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Tyr49-Ser356) of Human ST8SIA1 fused with a polyhistidine tag at the C-terminus.
Pureté
> 95 % as determined by reducing SDS-PAGE.
Stérilité
0.2 μm filtered
niveau d'endotoxine
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
Crystallography grade
ST8SIA1
Origine: Souris
Hôte: Cellules d'insectes
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
ELISA, WB, SDS, Crys
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl, pH 8.0.
Conseil sur la manipulation
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Stock
4 °C/-20 °C/-80 °C
Stockage commentaire
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Alpha-N-Acetylneuraminide alpha-2,8-Sialyltransferase (ST8SIA1) belongs to the glycosyltransferase 29 family. ST8SIA1 is a sialytransferase that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce GD3 and GT3. ST8SIA1 is highly expressed in melanoma cell lines, adult and fetal brain, low expressed in adult and fetal lung. ST8SIA1 may act as a type II transmembrane protein with a short N-termianl cytoplasmic domain and a single-pass transmembrane domain folowed by an enzymatic domain in the lument of the Golgi apparatus. Alternative Names: Alpha-N-Acetylneuraminide Alpha-2,8-Sialyltransferase, Alpha-2,8-Sialyltransferase 8A, Ganglioside GD3 Synthase, Ganglioside GT3 Synthase, Sialyltransferase 8A, SIAT8-A, Sialytransferase St8Sia I, ST8SiaI, ST8SIA1, SIAT8, SIAT8A