Recombinant Human High Mobility Group Protein B3/HMGB3 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Ala2-Glu200) of Human HMGB3 fused with a polyhistidine tag at the C-terminus.
Pureté
> 95 % as determined by reducing SDS-PAGE.
Stérilité
0.2 μm filtered
niveau d'endotoxine
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Conseil sur la manipulation
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Stock
4 °C/-20 °C/-80 °C
Stockage commentaire
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
HMG-2a Protein, HMG-4 Protein, HMG2A Protein, HMG4 Protein, Hmg2a Protein, Hmg4 Protein, RGD1564407 Protein, hmgb3 Protein, MGC54022 Protein, Xhmgb3 Protein, MGC88931 Protein, fa19b06 Protein, fj43d02 Protein, wu:fa19b06 Protein, wu:fj43d02 Protein, zgc:112073 Protein, HMGB3 Protein, NFD03 Protein, NFD3 Protein, NUCLEOSOME/CHROMATIN ASSEMBLY FACTOR Protein, high mobility group B3 Protein, HMG-1 Protein, HMG2a Protein, HMGB1 Protein, high mobility group box 3 Protein, high mobility group box 3b Protein, high mobility group box 3 S homeolog Protein, high mobility group protein Protein, high mobility group B3 Protein, High mobility group protein B3 Protein, HMGB3 Protein, Hmgb3 Protein, hmgb3 Protein, hmgb3b Protein, hmgb3.S Protein
Sub Type
Fusionprotein
Sujet
High Mobility Group Protein B3 (HMGB3) belongs to the HMGB family. Members of the HMG box subfamily are thought to be have an important role in DNA replication, nucleosome assembly and transcription. HMGB3 binds preferentiallly single-stranded DNA and unwinds double stranded DNA. HMGB3 consists of 200 amino acids and is localized to the cell nucleus. It contains two HMG box DNA-binding domain. HMGB3 binds preferentially single-stranded DNA and unwinds double stranded DNA. Alternative Names: High Mobility Group Protein B3, High Mobility Group Protein 2a, HMG-2a, High Mobility Group Protein 4, HMG-4, HMGB3, HMG2A, HMG4