Recombinant Human Endoplasmic Reticulum Mannosyl-Oligosaccharide 1,2-alpha-Mannosidase/MAN1B1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Asp106-Ala699) of Human MAN1B1 fused with a polyhistidine tag at the C-terminus.
Pureté
> 95 % as determined by reducing SDS-PAGE.
Stérilité
0.2 μm filtered
niveau d'endotoxine
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
MAN1B1
Origine: Rat
Hôte: Levure
Recombinant
> 90 %
ELISA
Restrictions
For Research Use only
Format
Liquid
Reconstitution
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Supplied as a 0.2 μm filtered solution of 50 mM TrisHCL,10 mM reduced Glutathione, pH 8.0.
Conseil sur la manipulation
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Stock
-80 °C
Stockage commentaire
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Date de péremption
6 months
Antigène
MAN1B1
(Mannosidase, Alpha, Class 1B, Member 1 (MAN1B1))
erman1 Protein, mana-er Protein, MAN1B1 Protein, ERMAN1 Protein, MANA-ER Protein, MRT15 Protein, E430019H13Rik Protein, ERMan1 Protein, Gm108 Protein, RGD1563595 Protein, mannosidase alpha class 1B member 1 Protein, mannosidase, alpha, class 1B, member 1 L homeolog Protein, endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase Protein, mannosidase, alpha, class 1B, member 1 Protein, MAN1B1 Protein, man1b1.L Protein, LOC480667 Protein, man1b1 Protein, Man1b1 Protein
Sub Type
Fusionprotein
Sujet
Endoplasmic Reticulum Mannosyl-Oligosaccharide 1,2-alpha-Mannosidase (MAN1B1) belongs to the glycosyl hydrolase 47 family. MAB1B1 is a single-pass type II membrane protein and widely expressed in many tissues. MAB1B1 is involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. MAB1B1 can be inhibited by both 1-deoxymannojirimycin (dMNJ) and kifunensine. Defects in MAN1B1 are the cause of mental retardation autosomal recessive type 15 (MRT15). Mental retardation is characterized by significantly below average general intellectual functioning, it is also associated with impairments in adaptative behavior and manifested during the developmental period. Alternative Names: Endoplasmic Reticulum Mannosyl-Oligosaccharide 1,2-Alpha-Mannosidase, ER Alpha-1,2-Mannosidase, ER Mannosidase 1, ERMan1, Man9GlcNAc2-Specific-Processing Alpha-Mannosidase, Mannosidase Alpha Class 1B Member 1, MAN1B1