Recombinant Human N-Acetyllactosaminide beta-1,3-N-Acetylglucosaminyltransferase/B3GNT1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Asp43-Cys415) of Human B3GNT1 fused with a polyhistidine tag at the C-terminus.
Pureté
> 95 % as determined by reducing SDS-PAGE.
Stérilité
0.2 μm filtered
niveau d'endotoxine
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Conseil sur la manipulation
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Stock
4 °C/-20 °C/-80 °C
Stockage commentaire
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
N-Acetyllactosaminide beta-1,3-N-Acetylglucosaminyltransferase (B3GNT1) is a member of the beta-1,3-N-Acetylglucosaminyltransferase family. B3GNT1 is a single-pass type II membrane protein and widely expressed in many tissues. B3GNT1 can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans. B3GNT1 is essential for the synthesis of poly-N-acetyllactosamine, a determinant for the blood group i antigen. It can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans. Alternative Names: N-Acetyllactosaminide Beta-1,3-N-Acetylglucosaminyltransferase, I-Beta-1,3-N-Acetylglucosaminyltransferase, iGnT, Poly-N-Acetyllactosamine Extension Enzyme, UDP-GlcNAc:BetaGal Beta-1,3-N-Acetylglucosaminyltransferase 1, B3GNT1, B3GNT6