LacI (AA 1-360) protein (His tag)

N° du produit ABIN7312770

Détail du produit

Antigène: LacI

Type de proteíne: Recombinant

Attributs du protein: AA 1-360

Origine: E. coli

Source: Escherichia coli (E. coli)

Purification/Conjugué: His tag

Application: ELISA, Mass Spectrometry (MS), SDS-PAGE (SDS), Western Blotting (WB)

Fonction: Recombinant E. coli LACI Protein is produced by our E. coli expression system and the target gene encoding Met1-Gln360 is expressed with a 6His tag at the C-terminus.

Séquence: MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ SLLIGVATSS LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS GLIINYPLDD QDAIAVEAAC TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ QIALLAGPLS SVSARLRLAG WHKYLTRNQI QPIAEREGDW SAMSGFQQTM QMLNEGIVPT AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS CYIPPLTTIK QDFRLLGQTS VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA RQVSRLESGQ HHHHHH

Pureté: Greater than 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Supplied as a 0.2 μM filtered solution of 20 mM Tris, 300 mM NaCl, 5 mM DTT, pH 8.0.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Détail du antigène

Antigène: LacI

Synonymes: ECK0342 Protein, JW0336 Protein, lactose-inducible lac operon transcriptional repressor Protein, lacI Protein

ID gène: 945007