Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
Specificité
Optimal preservation of protein structure, post-translational modifications and functions.
Attributs du produit
Recombinant human Interleukin-23 alpha / IL23A protein expressed in Insect cells.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Pureté
Purity is > 95 % , as determined by Coomassie stained SDS-PAGE.
niveau d'endotoxine
Less than 0.01 ng per μg protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 0.5 - 0.8 ng/ml, corresponding to a specific activity of 1.25-2.0 x 10^6 units/mg, determined by mouse splenocytes IL-17A secretion induced by mIL-23 in a dose dependent manner. Under this assay, the bioactivity is equivalent
IL23A
Origine: Souris
Hôte: Mammalian Cells
Recombinant
The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Restrictions
For Research Use only
Concentration
> 50 μg/mL
Buffer
1 x PBS
Stock
-80 °C
Stockage commentaire
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.