Functional Studies (Func), Standard (STD), Antibody Production (AbP), Protein Interaction (PI)
Specificité
Optimal preservation of protein structure, post-translational modifications and functions.
Attributs du produit
Recombinant human TGF-alpha (TGFA) (transcript variant 1) protein expressed in E.coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Pureté
> 98 % , as determined by Coomassie stained SDS-PAGE.
niveau d'endotoxine
Less than 0.01 ng per μg protein as determined by the LAL method.
Biological Activity Comment
The ED50 is 0.1 - 0.4 ng/ml, corresponding to a specific activity of 0.25 - 1.0 x 10^7 units/mg, determined by the dose dependent stimulation of BALB/c-3T3 mouse embryonic fibroblast cells proliferation.
TGFA
Origine: Macaque rhésus
Hôte: Escherichia coli (E. coli)
Recombinant
> 97 %
WB, SDS, Imm, PC
Indications d'application
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Restrictions
For Research Use only
Concentration
> 50 μg/mL
Buffer
1 x PBS
Stock
-80 °C
Stockage commentaire
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. [UniProtKB/Swiss-Prot Function]