Origine: Human Herpesvirus 5 (HHV-5), Human respiratory syncytial virus A
Hôte: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
WB
Indications d'application
This recombinant protein can be used for WB.
Restrictions
For Research Use only
Format
Lyophilized
Buffer
50 mM Tris, 100 mM Glycine, pH 7.5
Conseil sur la manipulation
Avoid repeated freeze-thaw cycles.
Stock
-20 °C,-80 °C
Stockage commentaire
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C.
Antigène
Glycoprotein / GP (Virus)
Classe de substances
Viral Protein
Sujet
Human cytomegalovirus is a species of the Cytomegalovirus genus of viruses, which in turn is a member of the viral family known as Herpesviridae or herpesviruses. It is typically abbreviated as HCMV or, commonly but more ambiguously, as CMV. CMV Virus Envelope Glycoportein B (CMV-GB) can be cleaved into glycoprotein GP55. Envelope glycoprotein that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. CMV-GB may be involved in the initial attachment via binding to heparan sulfate together with the gM/gN complex that binds heparin with higher affinity. Furthermore, CMV-GB can interact with host integrin ITGB1, PDGFRA and EGFR that likely serve as postattachment entry receptors. Also, CMV-GB participates in the fusion of viral and cellular membranes leading to virus entry into the host cell. Membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL.