AKT2 Protéine

N° du produit ABIN5624569

N° du produit (Fournisseur): 009-001-e71

Détail du produit

Antigène: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Application: Western Blotting (WB)

N° du produit (Fournisseur): 009-001-e71

Fournisseur: Rockland

Purification: Recombinant protein corresponds to amino acids 1 to 479 of mature human AKT2, Akt isoform 2. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.

Information d'application

Indications d'application:

Application Note: AKT2 (phosphatase treated) is suitable as a control for polyclonal or monoclonal anti-AKT2 in immunological assays. Akt2 recombinant protein is dephosphorylated and in an inactive state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized.

Western Blot Dilution: 50 ng

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.17 mg/mL

Buffer:

Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10 % glycerol

Stabilizer: 10 % (v/v) Glycerol

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 12 months

Détail du antigène

Antigène: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Autre désignation: AKT2 (AKT2 Produits)

ID gène: 208

UniProt: P31751

Pathways: Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Signalisation TLR, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling