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DBR1 Protein (AA 228-544) (His tag)
DBR1
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 90 % by SDS - PAGE
SDS
N° du produit ABIN5853256
Détail du produit
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Antigène
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DBR1
(Debranching Enzyme Homolog 1 (DBR1))
Type de proteíne
Recombinant
Attributs du protein
AA 228-544
Origine
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Humain
Source
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Escherichia coli (E. coli)
Purification/Conjugué
Cette DBR1 protéine est marqué à la His tag.
Application
SDS-PAGE (SDS)
Séquence
MGSSHHHHHH SSGLVPRGSH MGSHVKFAAL MQHQAKDKGQ TARATKFLAL DKCLPHRDFL QILEIEHDPS APDYLEYDIE WLTILRATDD LINVTGRLWN MPENNGLHAR WDYSATEEGM KEVLEKLNHD LKVPCNFSVT AACYDPSKPQ TQMQLIHRIN PQTTEFCAQL GIIDINVRLQ KSKEEHHVCG EYEEQDDVES NDSGEDQSEY NTDTSALSSI NPDEIMLDEE EDEDSIVSAH SGMNTPSVEP SDQASEFSAS FSDVRILPGS MIVSSDDTVD STIDREGKPG GTVESGNGED LTKVPLKRLS DEHEPEQRKK IKRRNQAIYA AVDDDDDDAA
Pureté
> 90 % by SDS - PAGE
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Stockage
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Format
Liquid
Concentration
0.5 mg/mL
Buffer
Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT.
Stock
4 °C,-20 °C,-80 °C
Stockage commentaire
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Détail du antigène
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Antigène
DBR1
(Debranching Enzyme Homolog 1 (DBR1))
Autre désignation
DBR1 (DBR1 Produits )
Synonymes
AW018415 Protein, wu:fc08f08 Protein, zgc:63930 Protein, ARABIDOPSIS THALIANA DEBRANCHING ENZYME 1 Protein, ATDBR1 Protein, F28M20.40 Protein, F28M20_40 Protein, debranching enzyme 1 Protein, dbr1 Protein, dbr1-a Protein, dbr1-b Protein, debranching RNA lariats 1 Protein, debranching enzyme homolog 1 (S. cerevisiae) Protein, Lariat debranching enzyme Protein, debranching enzyme 1 Protein, RNA lariat debranching enzyme Dbr1 Protein, debranching RNA lariats 1 S homeolog Protein, debranching RNA lariats 1 L homeolog Protein, DBR1 Protein, Dbr1 Protein, dbr1 Protein, dbr-1 Protein, dbr1.S Protein, dbr1.L Protein
Sujet
DBR1 is an RNA lariat debranching enzyme that hydrolyzes 2'-5' prime branched phosphodiester bonds. The encoded protein specifically targets the bonds at the branch point of excised lariat intron RNA, converting them to linear molecules that are then degraded. This protein may also be involved in retroviral replication. Recombinant human DBR1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Poids moléculaire
38 kDa (340aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
NCBI Accession
NP_057300
UniProt
Q9UK59
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