EIF3F Protein (AA 1-357) (His tag)
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- Antigène Voir toutes EIF3F Protéines
- EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-357
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette EIF3F protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Séquence
- MGSSHHHHHH SSGLVPRGSH MGSMATPAVP VSAPPATPTP VPAAAPASVP APTPAPAAAP VPAAAPASSS DPAAAAAATA APGQTPASAQ APAQTPAPAL PGPALPGPFP GGRVVRLHPV ILASIVDSYE RRNEGAARVI GTLLGTVDKH SVEVTNCFSV PHNESEDEVA VDMEFAKNMY ELHKKVSPNE LILGWYATGH DITEHSVLIH EYYSREAPNP IHLTVDTSLQ NGRMSIKAYV STLMGVPGRT MGVMFTPLTV KYAYYDTERI GVDLIMKTCF SPNRVIGLSS DLQQVGGASA RIQDALSTVL QYAEDVLSGK VSADNTVGRF LMSLVNQVPK IVPDDFETML NSNINDLLMV TYLANLTQSQ IALNEKLVNL
- Pureté
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product EIF3F Protéine
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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Denatured
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 0.4M urea
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Antigène
- EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))
- Autre désignation
- EIF3F (EIF3F Produits)
- Synonymes
- AO090005001590 Protein, Dmel\CG9769 Protein, eIF3-S5 Protein, eif3f Protein, LOC733116 Protein, eIF3f Protein, EIF3S5 Protein, eIF3-p47 Protein, 0610037M02Rik Protein, AA409853 Protein, Eif3s5 Protein, eukaryotic translation initiation factor 3 subunit F Protein, hypothetical protein Protein, CG9769 gene product from transcript CG9769-RA Protein, eukaryotic translation initiation factor 3 subunit 5 Protein, eukaryotic translation initiation factor 3, subunit F Protein, Eukaryotic translation initiation factor 3 subunit F Protein, eif3f Protein, ANI_1_120074 Protein, AOR_1_2780174 Protein, PTRG_03897 Protein, PAAG_00376 Protein, MCYG_08502 Protein, MGYG_08653 Protein, PGTG_04587 Protein, CG9769 Protein, LOC733116 Protein, EIF3F Protein, Eif3f Protein, eif-3.F Protein
- Sujet
- EIF3F is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF 2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AuG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Recombinant human EIF3F protein, fused to His-tag at N-terminus, was expressed in E.coli.
- Poids moléculaire
- 40.0kDa (380aa)
- NCBI Accession
- NP_003745
- UniProt
- O00303
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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