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ZWILCH Protein (AA 1-591) (His tag)
ZWILCH
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 85 % by SDS - PAGE
SDS
N° du produit ABIN5853741
Détail du produit
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Antigène
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ZWILCH
(Zwilch, Kinetochore Associated, Homolog (ZWILCH))
Type de proteíne
Recombinant
Attributs du protein
AA 1-591
Origine
Humain
Source
Toutes les sources à travers ZWILCH Protéines
Escherichia coli (E. coli)
Purification/Conjugué
Cette ZWILCH protéine est marqué à la His tag.
Application
SDS-PAGE (SDS)
Séquence
MGSSHHHHHH SSGLVPRGSH MGSMWERLNC AAEDFYSRLL QKFNEEKKGI RKDPFLYEAD VQVQLISKGQ PNPLKNILNE NDIVFIVEKV PLEKEETSHI EELQSEETAI SDFSTGENVG PLALPVGKAR QLIGLYTMAH NPNMTHLKIN LPVTALPPLW VRCDSSDPEG TCWLGAELIT TNNSITGIVL YVVSCKADKN YSVNLENLKN LHKKRHHLST VTSKGFAQYE LFKSSALDDT ITASQTAIAL DISWSPVDEI LQIPPLSSTA TLNIKVESGE PRGPLNHLYR ELKFLLVLAD GLRTGVTEWL EPLEAKSAVE LVQEFLNDLN KLDGFGDSTK KDTEVETLKH DTAAVDRSVK RLFKVRSDLD FAEQLWCKMS SSVISYQDLV KCFTLIIQSL QRGDIQPWLH SGSNSLLSKL IHQSYHGTMD TVSLSGTIPV QMLLEIGLDK LKKDYISFFI GQELASLNHL EYFIAPSVDI QEQVYRVQKL HHILEILVSC MPFIKSQHEL LFSLTQICIK YYKQNPLDEQ HIFQLPVRPT AVKNLYQSEK PQKWRVEIYS GQKKIKTVWQ LSDSSPIDHL NFHKPDFSEL TLNGSLEERI FFTNMVTCSQ VHFK
Pureté
> 85 % by SDS - PAGE
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Stockage
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Format
Liquid
Concentration
0.25 mg/mL
Buffer
Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 20 % glycerol, 1 mM DTT
Stock
4 °C,-20 °C,-80 °C
Stockage commentaire
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Détail du antigène
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Antigène
ZWILCH
(Zwilch, Kinetochore Associated, Homolog (ZWILCH))
Autre désignation
ZWILCH (ZWILCH Produits )
Synonymes
CG18639 Protein, CG18729 Protein, Dmel\\CG18729 Protein, Zwilch Protein, hzwilch Protein, kntc1ap Protein, KNTC1AP Protein, hZwilch Protein, 2310031L18Rik Protein, si:ch211-242m18.4 Protein, wu:fb10f09 Protein, zwilch kinetochore protein Protein, CG18729 gene product from transcript CG18729-RA Protein, protein zwilch homolog Protein, Zwilch Protein, zwilch Protein, LOC100374963 Protein, ZWILCH Protein
Sujet
ZWILCH belongs to the ZWILCH family. This protein is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. It is required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Recombinant human ZWILCH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Poids moléculaire
69.6kDa(614aa)
NCBI Accession
NP_060445
UniProt
Q9H900
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