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GARS Protein (AA 43-289) (His tag)
GARS
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 85 % by SDS - PAGE
SDS
N° du produit ABIN7280733
Détail du produit
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Antigène
Voir toutes GARS Protéines
GARS
(Glycyl-tRNA Synthetase (GARS))
Type de proteíne
Recombinant
Attributs du protein
AA 43-289
Origine
Toutes les origines à travers GARS Protéines
Humain
Source
Toutes les sources à travers GARS Protéines
Escherichia coli (E. coli)
Purification/Conjugué
Cette GARS protéine est marqué à la His tag.
Application
SDS-PAGE (SDS)
Séquence
MGSSHHHHHH SSGLVPRGSH MGSPISLPAA ASRSSMDGAG AEEVLAPLRL AVRQQGDLVR KLKEDKAPQV DVDKAVAELK ARKRVLEAKE LALQPKDDIV DRAKMEDTLK RRFFYDQAFA IYGGVSGLYD FGPVGCALKN NIIQTWRQHF IQEEQILEID CTMLTPEPVL KTSGHVDKFA DFMVKDVKNG ECFRADHLLK AHLQKLMSDK KCSVEKKSEM ESVLAQLDNY GQQELADLFV NYNVKSPITG NDLSPPVSFN LMFKTFIGPG
Pureté
> 85 % by SDS - PAGE
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Stockage
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Format
Liquid
Concentration
0.5 mg/mL
Buffer
Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
Stock
4 °C,-20 °C,-80 °C
Stockage commentaire
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Détail du antigène
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Antigène
GARS
(Glycyl-tRNA Synthetase (GARS))
Autre désignation
GARS (GARS Produits )
Synonymes
CMT2D Protein, DSMAV Protein, GlyRS Protein, HMN5 Protein, SMAD1 Protein, Aat-gly Protein, CG6778 Protein, Dmel\\CG6778 Protein, GRS Protein, gars Protein, team Protein, RGD1559871 Protein, GENA202 Protein, Gena201 Protein, Nmf249 Protein, Sgrp23 Protein, GB13467 Protein, MGC79495 Protein, fb02f03 Protein, si:dkey-276i5.1 Protein, wu:fb02f03 Protein, NI36_08435 Protein, glycyl-tRNA synthetase Protein, Glycyl-tRNA synthetase Protein, glycine--tRNA ligase Protein, glycyl-tRNA synthetase L homeolog Protein, Glycine--tRNA ligase Protein, GARS Protein, GlyRS Protein, Gars Protein, LOC408392 Protein, gars.L Protein, gars Protein, LOC692991 Protein, glyQ Protein, TTHA0543 Protein, NI36_RS08405 Protein
Sujet
GARS, also known as glycyl-tRNA synthetase, is one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. This enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Recombinant human GARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Poids moléculaire
30.0 kDa (270aa), confirmed by MALDI-TOF
NCBI Accession
NP_002038
UniProt
P41250
Pathways
Ribonucleoside Biosynthetic Process
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