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MTHFD2 Protein (AA 30-350) (His tag)
MTHFD2
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 95 % by SDS - PAGE
SDS
N° du produit ABIN5854086
Détail du produit
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Antigène
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MTHFD2
(Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 2, Methenyltetrahydrofolate Cyclohydrolase (MTHFD2))
Type de proteíne
Recombinant
Attributs du protein
AA 30-350
Origine
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Humain
Source
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Escherichia coli (E. coli)
Purification/Conjugué
Cette MTHFD2 protéine est marqué à la His tag.
Application
SDS-PAGE (SDS)
Séquence
MGSSHHHHHH SSGLVPRGSH MGSLAAVRNE AVVISGRKLA QQIKQEVRQE VEEWVASGNK RPHLSVILVG ENPASHSYVL NKTRAAAVVG INSETIMKPA SISEEELLNL INKLNNDDNV DGLLVQLPLP EHIDERRICN AVSPDKDVDG FHVINVGRMC LDQYSMLPAT PWGVWEIIKR TGIPTLGKNV VVAGRSKNVG MPIAMLLHTD GAHERPGGDA TVTISHRYTP KEQLKKHTIL ADIVISAAGI PNLITADMIK EGAAVIDVGI NRVHDPVTAK PKLVGDVDFE GVRQKAGYIT PVPGGVGPMT VAMLMKNTII AAKKVLRLEE REVLKSKELG VATN
Pureté
> 95 % by SDS - PAGE
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Stockage
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Format
Liquid
Concentration
1 mg/mL
Buffer
Liquid, In Phosphate buffered saline ( pH 7.4) containing 20 % glycerol, 1 mM DTT
Stock
4 °C,-20 °C,-80 °C
Stockage commentaire
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Détail du antigène
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Antigène
MTHFD2
(Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 2, Methenyltetrahydrofolate Cyclohydrolase (MTHFD2))
Autre désignation
MTHFD2 (MTHFD2 Produits )
Synonymes
nmdmc Protein, AW558851 Protein, NMDMC Protein, wu:fb38b11 Protein, wu:fe12e11 Protein, zgc:91891 Protein, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase L homeolog Protein, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase Protein, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 like S homeolog Protein, methylenetetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase Protein, mthfd2.L Protein, MTHFD2 Protein, mthfd2 Protein, mthfd2l.S Protein, Mthfd2 Protein
Sujet
MTHFD2 also known as Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial. MTHFD2 protein that possesses three distinct enzymatic activities, methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase and formate-tetrahydrofolate ligase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. Recombinant human MTHFD2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Poids moléculaire
37.2 kDa (344aa) confirmed by MALDI-TOF
NCBI Accession
NP_006627
UniProt
P13995
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