HMGCL Protein (AA 28-325) (His tag)

N° du produit ABIN5854983

Détail du produit

Antigène: HMGCL (3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL))

Type de proteíne: Recombinant

Attributs du protein: AA 28-325

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette HMGCL protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MTLPKRVKIV EVGPRDGLQN EKNIVSTPVK IKLIDMLSEA GLSVIETTSF VSPKWVPQMG DHTEVLKGIQ KFPGINYPVL TPNLKGFEAA VAAGAKEVVI FGAASELFTK KNINCSIEES FQRFDAILKA AQSANISVRG YVSCALGCPY EGKISPAKVA EVTKKFYSMG CYEISLGDTI GVGTPGIMKD MLSAVMQEVP LAALAVHCHD TYGQALANTL MALQMGVSVV DSSVAGLGGC PYAQGASGNL ATEDLVYMLE GLGIHTGVNL QKLLEAGNFI CQALNRKTSS KVAQATCKLH HHHHH

Pureté: > 90 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1ug of protein (determined by LAL method)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 20 % glycerol, 1  mM DTT.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: HMGCL (3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL))

Autre désignation: HMGCL (HMGCL Produits)

Synonymes: Afu7g01720 Protein, AO090038000541 Protein, AW476067 Protein, HL Protein, zgc:56248 Protein, 3-hydroxymethyl-3-methylglutaryl-CoA lyase S homeolog Protein, 3-hydroxymethyl-3-methylglutaryl-CoA lyase Protein, 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase Protein, 3-hydroxy-3-methylglutaryl-Coenzyme A lyase Protein, hmgcl.S Protein, HMGCL Protein, AFUA_7G01720 Protein, NFIA_114450 Protein, ACLA_065820 Protein, AOR_1_910074 Protein, PMAA_004600 Protein, TSTA_103060 Protein, ARB_04874 Protein, TRV_06573 Protein, Hmgcl Protein, hmgcl Protein

Sujet: HMGCL, also known as hydroxymethylglutaryl-CoA lyase, mitochondrial isoform 1, is a mitochondrial matrix protein that belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Multiple isoforms of the proteins are known due to alternative splicing. The major isoform (isoform 1) is most highly expressed in the liver whereas isoform 2 is found in energy-demanding tissues including the brain, heart, and skeletal muscle. Recombinant human HMGCL protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Poids moléculaire: 32.5kDa (305aa) 28-40KDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_000182

UniProt: P35914