CENPA Protein (His tag)

N° du produit ABIN620247

Détail du produit

Antigène: CENPA (Centromere Protein A (CENPA))

Type de proteíne: Recombinant

Origine: Humain

Hôte: Veuillez nous consulter SVP

Purification/Conjugué: Cette CENPA protéine est marqué à la His tag.

Application: Western Blotting (WB)

Attributs du produit: Recombinant Human Centromere Protein-A

Pureté: > 90 % as determined by SDS-PAGE.

Information d'application

Indications d'application: Western-Blot with monoclonal anti-hexa-His-tag antibody and Scleroderma patient sera. 0.15-0.4 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: CENPA is supplied in 20mM HEPES buffer pH-8, 100mM sodium chloride, and 6M Urea. Immunological functions 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

Stock: 4 °C

Détail du antigène

Antigène: CENPA (Centromere Protein A (CENPA))

Autre désignation: Centromere Protein-A (CENPA Produits)

Synonymes: CENP-A Protein, CenH3 Protein, Cenp-A Protein, cenpx Protein, cenp-a Protein, sim2 Protein, Cenp-a Protein, RGD1563607 Protein, CENPA Protein, centromere protein A Protein, centromere protein-A Protein, centromeric histone-3 like protein Protein, centromere-specific histone H3 CENP-A Protein, cenp-A Protein, centromere protein A L homeolog Protein, histone H3-like centromeric protein A Protein, CENPA Protein, Cenpa Protein, CENP-A Protein, cenpa Protein, cenp-A Protein, cnp1 Protein, HAN_3g422 Protein, Cenp-a Protein, cenpa.L Protein, CMU_016480 Protein

Sujet: CENPA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 17,015 Dalton. CENPA is expressed with a -6x His tag and purified by proprietary chromatographic techniques. Introduction: Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENPA to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies. CENPA is another important centromeric autoantigen in addition to CENPB: it has a molecular weight of approx. 20 kDa and is incorporated into centromeric chromatin due to its histone-like properties. CENPA antibodies are an important marker for correct diagnosis of Scleroderma / CREST syndrome in CENPB-autoantibody negative patients. Synonyms: Histone H3-like centromeric protein A, Centromere protein A, CENP-A, Centromere autoantigen A, CENPA.

Pathways: Chromatin Binding, Maintenance of Protein Location