> 95.0 % as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Restrictions
For Research Use only
Format
Lyophilized
Buffer
The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate.It is recommended to reconstitute the lyophilized LDH in sterile 18M -cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
The DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart. Introduction: Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals. A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist. Synonyms: Lactate Dehydrogenase, LDH.