FNDC5
(Fibronectin Type III Domain Containing 5 (FNDC5))
Activité biologique
Active
Attributs du protein
AA 2-137
Origine
Humain
Source
Escherichia coli (E. coli)
Purification/Conjugué
Cette FNDC5 protéine est marqué à la His tag.
Application
Activity Assay (AcA), Cell Culture (CC)
Attributs du produit
Tag location: N-terminal His Tag
Pureté
> 90 %
Biological Activity Comment
Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. It was reported that FNDC5 significantly decreased cell number, migration and viability through apoptosis in malignant MDA-MB-231 cells. Thus MDA-MB-231 cells were seeded overnight at a density of 5,000 cells/well, and treated with or without various concentrations of FNDC5 for 48h, then MDA-MB-231 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC. Cell apoptosis of MDA-MB-231 cells after incubation with FNDC5 for 48h observed by inverted microscope was shown in Figure 1.