IL17 Receptor B Protein (AA 61-200) (His tag)

N° du produit ABIN6239810

Détail du produit

Antigène: IL17 Receptor B (IL17RB) (Interleukin 17 Receptor B (IL17RB))

Activité biologique: Active

Attributs du protein: AA 61-200

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette IL17 Receptor B protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 80 %

Biological Activity Comment: IL17RB (Interleukin-17 receptor B) is a receptor for the proinflammatory cytokines IL17B (Interleukin-17B) and IL17E (Interleukin-17E). This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. Thus a binding ELISA assay was constructed to detect the association of recombinant human IL17RB with recombinant human IL17B. Briefly, IL17RB were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17B-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17RB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RB with IL17B was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of IL17RB with IL17B.

Information d'application

Indications d'application: Isoelectric Point: 8.9

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: IL17 Receptor B (IL17RB) (Interleukin 17 Receptor B (IL17RB))

Abstract: IL17RB Produits

Synonymes: IL17RB Protein, CRL4 Protein, EVI27 Protein, IL17BR Protein, IL17RH1 Protein, Evi27 Protein, IL-17ER Protein, IL-17Rh1 Protein, Il17br Protein, interleukin 17 receptor B Protein, IL17RB Protein, Il17rb Protein

Sujet: Alternative Names: IL17R-B, CRL4, EVI27, IL17BR, IL17RH1, Cytokine receptor-like 4, IL-17 receptor homolog 1

Poids moléculaire: 19kDa

UniProt: Q9NRM6