GSTP1 Protein (AA 1-210) (His tag)

N° du produit ABIN6239834

Détail du produit

Antigène: GSTP1 (Glutathione S-Transferase pi 1 (GSTP1))

Activité biologique: Active

Attributs du protein: AA 1-210

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette GSTP1 protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 95 %

Biological Activity Comment: GSTp (Glutathione S-transferase P) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 is identified as a CDK5 (Cyclin dependent kinase-5) regulatory protein, and is thought to regulates negatively CDK5 activity via p25/p35 translocation. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human GSTp and recombinant human CDK5. Briefly, GSTp were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CDK5-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GSTp pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of GSTp and CDK5 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of GSTp with CDK5.

Information d'application

Indications d'application: Isoelectric Point: 6.2

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: GSTP1 (Glutathione S-Transferase pi 1 (GSTP1))

Autre désignation: Glutathione S Transferase Pi (GSTp) (GSTP1 Produits)

Synonymes: DFN7 Protein, FAEES3 Protein, GST3 Protein, GSTP Protein, PI Protein, GST-P Protein, Gst3 Protein, Gstp Protein, Gstp2 Protein, gst-Pi Protein, GstpiB Protein, glutathione S-transferase P-like Protein, glutathione S-transferase pi 1 Protein, glutathione S-transferase pi 1 L homeolog Protein, glutathione S-transferase, pi 1 Protein, LOC100739163 Protein, GSTP1 Protein, Gstp1 Protein, LOC100861197 Protein, gstp1.L Protein

Sujet: Alternative Names: GSTP1, GST-P1, DFN7, FAEES3, GST3

Poids moléculaire: 25kDa

UniProt: P09211

Pathways: Cellular Response to Molecule of Bacterial Origin