GLO1 Protein (AA 1-184)

N° du produit ABIN6387621

Détail du produit

Antigène: GLO1 (Glyoxalase I (GLO1))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-184

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Séquence: ARPCIPKSFG YSSVVCVCNA TYCDSFDPPT FPALGTFSRY ESTRSGRRME LSMGPIQANH TGTGLLLTLQ PEQKFQKVKG FGGAMTDAAA LNILALSPPA QNLLLKSYFS EEGIGYNIIR VPMASCDFSI RTYTYADTPD DFQLHNFSLP EEDTKLKIPL IHRALQLAQR PVSLLASPWT SPTWLKTNGA VNGKGSLKGQ PGDIYHQTWA RYFVKFLDAY AEHKLQFWAV TAENEPSAGL LSGYPFQCLG FTPEHQRDFI ARDLGPTLAN STHHNVRLLM LDDQRLLLPH WAKVVLTDPE AAKYVHGIAV HWYLDFLAPA KATLGETHRL FPNTMLFASE ACVGSKFWEQ SVRLGSWDRG MQYSHSIITN LLYHVVGWTD WNLALNPEGG PNWVRNFVDS PIIVDITKDT FYKQPMFYHL GHFSKFIPEG SQRVGLVASQ KNDLDAVALM HPDGSAVVVV LNRSSKDVPL TIKDPAVGFL ETISPGYSIH TYLWRRQHHH HHH

Pureté: > 90 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Specific activity: > 400 units/mg. One unit will form 1.0umol of S-lactoylgutathione from methylglyoxal and reduced glutathione per minute at pH6.5 at 25C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20 mM Tris-HCl buffer (  pH 8.0) containing 1 mM DTT, 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: GLO1 (Glyoxalase I (GLO1))

Autre désignation: GLO1 (GLO1 Produits)

Synonymes: GLOD1 Protein, GLYI Protein, 0610009E22Rik Protein, 1110008E19Rik Protein, 2510049H23Rik Protein, AW550643 Protein, GLY1 Protein, Glo-1 Protein, Glo-1r Protein, Glo-1s Protein, Glo1-r Protein, Glo1-s Protein, Qglo Protein, cb554 Protein, zgc:66035 Protein, wu:fb82g09 Protein, glod1 Protein, glyi Protein, glo1 Protein, GLO1 Protein, ATGLX1 Protein, F12F1.32 Protein, F12F1_32 Protein, GLYOXALASE I Protein, glyoxalase I homolog Protein, trypanothione-dependent glyoxalase I Protein, glyoxalase I Protein, glyoxalase 1 Protein, glyoxalase 1 L homeolog Protein, glyoxalase 1 S homeolog Protein, lactoylglutathione lyase Protein, glyoxalase/bleomycin resistance protein/dioxygenase superfamily protein Protein, GLO1 Protein, Glo1 Protein, glo1 Protein, glo1.L Protein, glo1.S Protein, GST3 Protein, GLX1 Protein, STY1687 Protein, Bcen_2094 Protein, gloA Protein

Sujet: Lactoylglutathione lyase, also known as GLO1, belongs to the glyoxalaseIfamily. Glyoxalase I is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione.This enzyme is ubiquitously expressed and is also present in many tumor cell lines, in which its concentration is often upregulated. Recombinant human GLO1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 20.7 kDa (184 aa), confirmed by MALDI-TOF

NCBI Accession: NP_006699

UniProt: Q04760