NME2 Protein (AA 1-152)

N° du produit ABIN6387709

Détail du produit

Antigène: NME2 (Nucleoside diphosphate kinase B (NME2))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-152

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Séquence: MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE

Pureté: > 90 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 1,800 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20 mM Tris-HCl buffer (  pH 8.0) containing 1  mM DTT, 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: NME2 (Nucleoside diphosphate kinase B (NME2))

Autre désignation: NME2 (NME2 Produits)

Synonymes: NDKB Protein, NDPK-B Protein, NDPKB Protein, NM23-H2 Protein, NM23B Protein, PUF Protein, nm23-2 Protein, p18-12d Protein, nm23-M2 Protein, NM23-C2 Protein, NME/NM23 nucleoside diphosphate kinase 2 Protein, non-metastatic cells 2, protein (NM23B) expressed in Protein, NME2 Protein, Nme2 Protein

Sujet: NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. Recombinant human NME2 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 17.2 kDa (152aa), confirmed by MALDI-TOF

NCBI Accession: NP_001018149

UniProt: P22392