ECHS1 Protein (AA 28-290) (His tag)
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- Antigène Voir toutes ECHS1 Protéines
- ECHS1 (Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- AA 28-290
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette ECHS1 protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Séquence
- MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ
- Pureté
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 150 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25C.
- Top Product
- Discover our top product ECHS1 Protéine
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 20 % glycerol, 100 mM NaCl
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Antigène
- ECHS1 (Enoyl CoA Hydratase, Short Chain, 1, Mitochondrial (ECHS1))
- Autre désignation
- ECHS1 (ECHS1 Produits)
- Synonymes
- SCEH Protein, cOR6not Protein, fj55e05 Protein, si:zc217g15.1 Protein, wu:fj55e05 Protein, C80529 Protein, enoyl-CoA hydratase, short chain, 1, mitochondrial L homeolog Protein, enoyl-CoA hydratase, short chain 1 Protein, enoyl CoA hydratase, short chain, 1, mitochondrial Protein, enoyl Coenzyme A hydratase, short chain, 1, mitochondrial Protein, echs1.L Protein, ECHS1 Protein, Echs1 Protein, echs1 Protein
- Sujet
- Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Poids moléculaire
- 30.6 kDa (284aa), confirmed by MALDI-TOF
- Pathways
- Monocarboxylic Acid Catabolic Process
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