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DPH2 Protein (AA 1-489) (His tag)
DPH2
Origine: Humain
Hôte: Baculovirus infected Insect Cells
Recombinant
> 85 % by SDS - PAGE
SDS
N° du produit ABIN6388024
Détail du produit
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Antigène
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DPH2
(Diphthamide Biosynthesis Protein 2 (DPH2))
Type de proteíne
Recombinant
Attributs du protein
AA 1-489
Origine
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Humain
Source
Toutes les sources à travers DPH2 Protéines
Baculovirus infected Insect Cells
Purification/Conjugué
Cette DPH2 protéine est marqué à la His tag.
Application
SDS-PAGE (SDS)
Séquence
MESMFSSPAE AALQRETGVP GLLTPLPDLD GVYELERVAG FVRDLGCERV ALQFPDQLLG DAVAVAARLE ETTGSKMFIL GDTAYGSCCV DVLGAEQAGA QALIHFGPAC LSPPARPLPV AFVLRQRSVA LELCVKAFEA QNPDPKAPVV LLSEPACAHA LEALATLLRP RYLDLLVSSP AFPQPVGSLS PEPMPLERFG RRFPLAPGRR LEEYGAFYVG GSKASPDPDL DPDLSRLLLG WAPGQPFSSC CPDTGKTQDE GARAGRLRAR RRYLVERARD ARVVGLLAGT LGVAQHREAL AHLRNLTQAA GKRSYVLALG RPTPAKLANF PEVDVFVLLA CPLGALAPQL SGSFFQPILA PCELEAACNP AWPPPGLAPH LTHYADLLPG SPFHVALPPP ESELWETPDV SLITGDLRPP PAWKSSNDHG SLALTPRPQL ELAESSPAAS FLSSRSWQGL EPRLGQTPVT EAVSGRRGIA IAYEDEGSGL EHHHHHH
Pureté
> 85 % by SDS - PAGE
niveau d'endotoxine
< 1.0 EU per 1 microgram of protein (determined by LAL method)
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Alternatives
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Stockage
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Format
Liquid
Concentration
0.5 mg/mL
Buffer
Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 1 mM DTT, 20 % glycerol.
Stock
4 °C,-20 °C,-80 °C
Stockage commentaire
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Détail du antigène
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Antigène
DPH2
(Diphthamide Biosynthesis Protein 2 (DPH2))
Autre désignation
DPH2 (DPH2 Produits )
Synonymes
DPH2L2 Protein, 9130020C19Rik Protein, AI467389 Protein, Dph2l2 Protein, id:ibd5058 Protein, zgc:162269 Protein, DPH2 homolog Protein, DPH2 homolog (S. cerevisiae) Protein, hypothetical protein Protein, DPH2 Protein, Dph2 Protein, dph2 Protein, CAALFM_C400690CA Protein
Sujet
DPH2, also known as diphthamide biosynthesis protein 2, is a homodimer and each of its monomers can bind a [4Fe-4S] cluster. It is the target of ADP ribosylating diphtheria toxin (DT) and Pseudomonas exotoxin A (PE). It was identified by its ability to complement a diphthamide mutant strain, and thus functions in diphthamide biosynthesis. Its loss pre-activates NF-kB and death receptor pathways and renders MCF7 cells hypersensitive to tumor necrosis factor. Recombinant human DPH2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Poids moléculaire
53.1kDa (497aa) 50-70kDa (SDS-PAGE under reducing conditions)
NCBI Accession
NP_001375
UniProt
Q9BQC3
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