MGAT2 Protein (AA 30-447) (His tag)

N° du produit ABIN6388196

Détail du produit

Antigène: MGAT2 (Mannosyl (Alpha-1,6-)-Glycoprotein beta-1,2-N-Acetylglucosaminyltransferase (MGAT2))

Type de proteíne: Recombinant

Attributs du protein: AA 30-447

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette MGAT2 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: ADPRQRKNEA LAPPLLDAEP ARGAGGRGGD HPSVAVGIRR VSNVSAASLV PAVPQPEADN LTLRYRSLVY QLNFDQTLRN VDKAGTWAPR ELVLVVQVHN RPEYLRLLLD SLRKAQGIDN VLVIFSHDFW STEINQLIAG VNFCPVLQVF FPFSIQLYPN EFPGSDPRDC PRDLPKNAAL KLGCINAEYP DSFGHYREAK FSQTKHHWWW KLHFVWERVK ILRDYAGLIL FLEEDHYLAP DFYHVFKKMW KLKQQECPEC DVLSLGTYSA SRSFYGMADK VDVKTWKSTE HNMGLALTRN AYQKLIECTD TFCTYDDYNW DWTLQYLTVS CLPKFWKVLV PQIPRIFHAG DCGMHHKKTC RPSTQSAQIE SLLNNNKQYM FPETLTISEK FTVVAISPPR KNGGWGDIRD HELCKSYRRL QHHHHHH

Pureté: > 90 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1ug of protein (determined by LAL method)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl (  pH 7.5) containing 10 % glycerol, 0.15M NaCl, 1  mM DTT.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: MGAT2 (Mannosyl (Alpha-1,6-)-Glycoprotein beta-1,2-N-Acetylglucosaminyltransferase (MGAT2))

Autre désignation: MGAT2 (MGAT2 Produits)

Synonymes: CDG2A Protein, CDGS2 Protein, GLCNACTII Protein, GNT-II Protein, GNT2 Protein, CG7921 Protein, Dmel\\CG7921 Protein, GlcNAc-TII Protein, GlcNAcT2 Protein, dMGAT2 Protein, MGC89475 Protein, Gnt2 Protein, AA407964 Protein, CH73-301C19.1 Protein, zgc:162268 Protein, mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase Protein, CG7921 gene product from transcript CG7921-RB Protein, mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase L homeolog Protein, mannoside acetylglucosaminyltransferase 2 Protein, MGAT2 Protein, Mgat2 Protein, mgat2.L Protein, mgat2 Protein

Sujet: MGAT2, also known as alpha-1, 6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, is a single-pass type 2 membrane protein. It has the typical glycosyltransferase domains that composes a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. This protein catalyzes an essential step in the conversion of oligo-mannose to complex N-glycans. Sometimes mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. Recombinant human MGAT2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Poids moléculaire: 49.3kDa (427aa) 40-57kDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_002399

UniProt: Q10469