Biglycan Protein (BGN) (AA 38-368) (His tag)

N° du produit ABIN7529401

Détail du produit

Antigène: Biglycan (BGN)

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 38-368

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette Biglycan protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: ADPDEEASGA DTSGVLDPDS VTPTYSAMCP FGCHCHLRVV QCSDLGLKSV PKEISPDTTL LDLQNNDISE LRKDDFKGLQ HLYALVLVNN KISKIHEKAF SPLRKLQKLY ISKNHLVEIP PNLPSSLVEL RIHDNRIRKV PKGVFSGLRN MNCIEMGGNP LENSGFEPGA FDGLKLNYLR ISEAKLTGIP KDLPETLNEL HLDHNKIQAI ELEDLLRYSK LYRLGLGHNQ IRMIENGSLS FLPTLRELHL DNNKLARVPS GLPDLKLLQV VYLHSNNITK VGVNDFCPMG FGVKRAYYNG ISLFNNPVPY WEVQPATFRC VTDRLAIQFG NYKKHHHHHH

Pureté: > 90 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured in inhibit the cell growth using 3T3-L1 mouse embryonic fibroblast adipose-like cells. The ED50 for this effect is less or equal to 20 ug/ml.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: Biglycan (BGN)

Autre désignation: BGN (BGN Produits)

Synonymes: DSPG1 Protein, PG-S1 Protein, PGI Protein, SLRR1A Protein, BG Protein, BSPG1 Protein, bgn Protein, zgc:91989 Protein, dspg1 Protein, pg-s1 Protein, slrr1a Protein, xbgn Protein, BGN Protein, zgc:77123 Protein, DKFZp468I2427 Protein, biglycan Protein, biglycan L homeolog Protein, biglycan a Protein, biglycan S homeolog Protein, biglycan b Protein, BGN Protein, Bgn Protein, bgn.L Protein, bgna Protein, bgn.S Protein, bgn Protein, bgnb Protein

Sujet: BGN, also known as biglycan preproprotein, is a small leucine-rich repeat proteoglycan (SLRP). It plays an important role in the development and maintenance of many tissues. Core protein of this protein binds to the growth factors BMP-4 and influences its bioactivity. This protein is also involved in collagen fiber assembly. Recombinant human BGN protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Poids moléculaire: 38.3kDa (340aa) 40-57KDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_001702

UniProt: P21810

Pathways: Glycosaminoglycan Metabolic Process