IL-34 Protein (AA 21-242) (His tag)

N° du produit ABIN7281356

Détail du produit

Antigène: IL-34 (IL34) (Interleukin 34 (IL34))

Type de proteíne: Recombinant

Attributs du protein: AA 21-242

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette IL-34 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: ADPNEPLEMW PLTQNEECTV TGFLRDKLQY RSRLQYMKHY FPINYKISVP YEGVFRIANV TRLQRAQVSE RELRYLWVLV SLSATESVQD VLLEGHPSWK YLQEVQTLLL NVQQGLTDVE VSPKVESVLS LLNAPGPNLK LVRPKALLDN CFRVMELLYC SCCKQSSVLN WQDCEVPSPQ SCSPEPSLQY AATQLYPPPP WSPSSPPHST GSVRPVRAQG EGLLPHHHHH H

Pureté: > 90 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1ug of protein (determined by LAL method)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-Hcl buffer (  pH 8.5) containing 1  mM DTT,0.15M NaCl, 01  mM PMSF, 1  mM EDTA, 30 % glycerol.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: IL-34 (IL34) (Interleukin 34 (IL34))

Autre désignation: IL34 (IL34 Produits)

Synonymes: C16orf77 Protein, IL-34 Protein, 2010004A03Rik Protein, AI593503 Protein, C18H16ORF77 Protein, interleukin 34 Protein, IL34 Protein, Il34 Protein

Sujet: IL34, also known as interleukin-34 isoform 1, is a secreted cytokine. This protein is expressed in a variety of tissues richest in the spleen and stimulates the proliferation of monocytes. In a functional study, IL-34, such as CSF-1, another ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1 / 2) in human monocytes. In addition, it promoted the formation of macrophage precursor colonization units-macrophages (CFU-M) in human bone marrow cultures. Recombinant human IL34, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Poids moléculaire: 26.3kDa (231aa) 28-40kDa (SDS-PAGE under reducing conditions)

NCBI Accession: NP_689669

UniProt: Q6ZMJ4

Pathways: Signalisation RTK