DnaK (AA 385-546) Protéine

N° du produit ABIN666828

Détail du produit

Antigène: DnaK

Type de proteíne: Recombinant

Attributs du protein: AA 385-546

Origine: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Attributs du produit: Dnak(385-546), E.coli, Recombinant, E.coli

Pureté: > 95 % by SDS - PAGE

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid. 25 mM Tris-HCl, pH7.5, 2 mM beta-mercaptoethanol, 1 mM EDTA.

Stock: 4 °C

Détail du antigène

Antigène: DnaK

Autre désignation: Dnak (385-546)

Sujet: DNAK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid(Met) is attached at N- terminus. Synonyms: Chaperone protein dnaK, HSP70, groP, grpF, seg, Heat shock protein 70, Chaperone Hsp70, Co chaperone with DnaJ, dnaK, Heat shock 70 kDa protein,. NCBI no.: NP_414555

Poids moléculaire: 17.7kDa (163aa)