Follistatin Protein (FST)

N° du produit ABIN6699882

N° du produit (Fournisseur): 010-001-u88-0005

Détail du produit

Antigène: Follistatin (FST)

Type de proteíne: Recombinant

Origine: Souris

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

N° du produit (Fournisseur): 010-001-u88-0005

Fournisseur: Rockland

Fonction: Mouse Follistatin Recombinant Protein

Purification: Follistatin purity was determined to be greater than 90% as determined by analysis by reducing and non-reducing SDS-pAGE.

Pureté: 90,00%

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent neutralization of 7.5 ng/mL human Activin A. Complete neutralization is typically reached at less than 0.3 ug/mL.

Information d'application

Indications d'application:

Other: User Optimized

Application_Note: Follistatin Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Follistatin in immunological assays.

Commentaires:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5 μL (5-50 μL)

Buffer: Buffer Formulation: 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: Follistatin (FST)

Autre désignation: Fst (FST Produits)

Sujet:

Synonyms: FS, activin-binding protein, FSH-suppressing protein (FSP)

Background: Follistatin is an autocrine acting protein that is expressed by many tissues, but at noteably higher levels in the ovary and skin. Follistatin functions to negatively regulate the signaling of a wide variety of TGF-beta family members (activin, BMPs, myostatin, GDF-11 and TGF-beta 1). Mechanistically, follistatin works as an antagonist by complexing with TGF-beta family members to prevent them from interacting with their signaling receptors. Recombinant mouse follistatin is a non-glycosylated protein, containing 289 amino acids, with a molecular weight of 31.6 kDa.

UniProt: P47931

Pathways: Negative Regulation of Hormone Secretion