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IFNG1-2 Protéine

Rockland 012-001-v20-0020 IFNG1-2 Origine: Rat Hôte: Escherichia coli (E. coli) Recombinant 95,00% SDS
Rockland
N° du produit ABIN6699981
N° du produit (Fournisseur): 012-001-v20-0020
  • Antigène Tous les produits IFNG1-2
    IFNG1-2 (Interferon, gamma 1-2 (IFNG1-2))
    Type de proteíne
    Recombinant
    Origine
    • 2
    • 1
    Rat
    Source
    • 3
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    N° du produit (Fournisseur)
    012-001-v20-0020
    Fournisseur
    Rockland
    Fonction
    Rat Interferon gamma Recombinant Protein
    Purification
    Interferon gamma purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
    Pureté
    95,00%
    niveau d'endotoxine
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    The activity is determined in a viral challenge assay using EMC virus on L929 and is typically less than 0.1 ng/mL.
  • Indications d'application

    Other: User Optimized

    Application_Note: Interferon-gamma Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interferon-gamma in immunological assays.

    Commentaires

    Suggested_Applications: Cellular Assay

    Other_Performance_Data:

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 20 μL (20-200 μL)

    Concentration
    0.1 mg/mL
    Buffer
    Lyophilized in 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5.
    Agent conservateur
    Without preservative
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Date de péremption
    6 months
  • Antigène
    IFNG1-2 (Interferon, gamma 1-2 (IFNG1-2))
    Autre désignation
    INF gamma (IFNG1-2 Produits)
    Sujet

    Synonyms: Type II interferon, T cell interferon, Immune Interferon, MAF

    Background: Interferon gamma, IFNγ, is a major immune-modulating molecule produced mainly by T cells and natural killer cells activated by antigens, mitogens or alloantigens. Most immune cells express IFNγ receptors and respond to IFNγ-induced signaling by up-regulating MHC class I expression. Recombinant rat IFNγ is a non-glycosylated protein, containing 135 amino acids, with a molecular weight of 15.6 kDa.

    UniProt
    P01581
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