Leptin Protein (LEP)

N° du produit ABIN6700213

N° du produit (Fournisseur): 012-001-320-0200

Détail du produit

Antigène: Leptin (LEP)

Type de proteíne: Recombinant

Origine: Rat

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

N° du produit (Fournisseur): 012-001-320-0200

Fournisseur: Rockland

Fonction: Rat Leptin Recombinant Protein

Purification: Leptin purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Pureté: 95,00%

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is currently undetermined.

Information d'application

Indications d'application:

Other: User Optimized

Application_Note: Leptin Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Leptin in immunological assays.

Commentaires:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: Leptin (LEP)

Autre désignation: Lep (LEP Produits)

Sujet:

Synonyms: Obesity protein

Background: Leptin is a protein that is thought to have a critical role in the physiologic regulation of body weight via its capacity to inhibit food intake and stimulate energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses. Recombinant rat Leptin is a non-glycosylated protein, containing 147 amino acids, with a molecular weight of 16.2 kDa.

UniProt: P50596

Pathways: Signalistation JAK/STAT, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process