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Leptin Protein (LEP)

Rockland 012-001-320-0200 LEP Origine: Rat Hôte: Escherichia coli (E. coli) Recombinant 95,00% SDS
Rockland
N° du produit ABIN6700213
N° du produit (Fournisseur): 012-001-320-0200
  • Antigène Voir toutes Leptin (LEP) Protéines
    Leptin (LEP)
    Type de proteíne
    Recombinant
    Origine
    • 12
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Source
    • 30
    • 20
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    N° du produit (Fournisseur)
    012-001-320-0200
    Fournisseur
    Rockland
    Fonction
    Rat Leptin Recombinant Protein
    Purification
    Leptin purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
    Pureté
    95,00%
    niveau d'endotoxine
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    The activity is currently undetermined.
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    Discover our top product LEP Protéine
  • Indications d'application

    Other: User Optimized

    Application_Note: Leptin Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Leptin in immunological assays.

    Commentaires

    Suggested_Applications: Cellular Assay

    Other_Performance_Data:

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 100 μL

    Concentration
    0.1 mg/mL
    Buffer

    Buffer: 0.1 % Trifluoroacetic acid

    Stabilizer: None

    Agent conservateur
    Without preservative
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Date de péremption
    6 months
  • Antigène
    Leptin (LEP)
    Autre désignation
    Lep (LEP Produits)
    Sujet

    Synonyms: Obesity protein

    Background: Leptin is a protein that is thought to have a critical role in the physiologic regulation of body weight via its capacity to inhibit food intake and stimulate energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses. Recombinant rat Leptin is a non-glycosylated protein, containing 147 amino acids, with a molecular weight of 16.2 kDa.

    UniProt
    P50596
    Pathways
    Signalistation JAK/STAT, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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