SARS-CoV-2 Spike S2 Protein (His tag)

N° du produit ABIN6952626

Détail du produit

Antigène: SARS-CoV-2 Spike S2

Type de proteíne: Recombinant

Origine: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purification/Conjugué: Cette SARS-CoV-2 Spike S2 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Fonction: SARS-CoV-2 (COVID-19) S2 protein, His Tag

Séquence: AA 686-1213

Attributs du produit: SARS-CoV-2 S2 protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1). Predicted N-terminus: Ser 686
This protein carries a polyhistidine tag at the C-terminus.

Pureté: >95 % as determined by SDS-PAGE.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.

Référence

Longworth, Dittmar: "An antigen microarray protocol for COVID-19 serological analysis." dans: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).

Tosif, Neeland, Sutton, Licciardi, Sarkar, Selva, Do, Donato, Quan Toh, Higgins, Van de Sandt, Lemke, Lee, Shoffner, Flanagan, Arnold, Mordant, Mulholland, Bines, Dohle, Pellicci, Curtis, McNab et al.: "Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19. ..." dans: Nature communications, Vol. 11, Issue 1, pp. 5703, (2020) (PubMed).

Wei, Wan, Yan, Wang, Zhang, Yang, Zhang, Fan, Li, Deng, Sun, Gong, Yang, Wang, Wang, Li, Yang, Li, Zhang, Wang, Du, Zong, Yin, Zhang, Wang, Peng, Lin, Feng, Qin, Chen, Gao, Zhang, Cao, Zhong: "HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry." dans: Nature metabolism, Vol. 2, Issue 12, pp. 1391-1400, (2020) (PubMed).

Détail du antigène

Antigène: SARS-CoV-2 Spike S2

Abstract: SARS-CoV-2 Spike S2 Produits

Synonymes: E2 Protein, Surface Glycoprotein Protein, S Protein

Classe de substances: Viral Protein

Sujet: It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Poids moléculaire: 60.0 kDa

ID gène: 43740568

UniProt: P0DTC2