Cette SARS-CoV-2 Spike S1 protéine est marqué à la His tag.
Application
SDS-PAGE (SDS), ELISA
Fonction
SARS-CoV-2 (COVID-19) S protein RBD (W436R), His Tag
Séquence
AA 319-541
Attributs du produit
SARS-CoV-2 S protein RBD (W436R), His Tag (SPD-S52H7) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1 (W436R). Predicted N-terminus: Arg 319 This protein carries a polyhistidine tag at the C-terminus. The W436R mutation in the RBD of Spike protein shows a higher binding affinity to human ACE2.
Origine: SARS Coronavirus-2 (SARS-CoV-2)
Hôte: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Lyophilized
Buffer
PBS, pH 7.4
Conseil sur la manipulation
Please avoid repeated freeze-thaw cycles.
Stock
4 °C,-20 °C,-80 °C
Stockage commentaire
For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable after storage at: 4-8°C for 12 months in lyophilized state, -70°C for 3 years under sterile conditions after reconstitution.
E2 Protein, Surface Glycoprotein Protein, S Protein
Classe de substances
Viral Protein
Sujet
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.