Cette SARS-CoV-2 NSP1 protéine est marqué à la His tag.
Application
SDS-PAGE (SDS)
Fonction
SARS-CoV-2 (COVID-19) NSP1 Protein, His Tag
Séquence
AA 1-180
Attributs du produit
SARS-CoV-2 NSP1, His Tag is expressed from E.coli cells. It contains AA Met 1 - Gly 180 (Accession # YP_009725297.1). Predicted N-terminus: Met This protein carries a polyhistidine tag at the C-terminus.
Pureté
>90 % as determined by SDS-PAGE.
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1.0 EU per μg by the LAL method.
Indications d'application
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Lyophilized
Buffer
PBS, Arginine, pH 7.4
Stock
4 °C,-20 °C,-80 °C
Stockage commentaire
For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable after storage at: 4-8°C for 12 months in lyophilized state, -70°C for 3 years under sterile conditions after reconstitution.
pp1ab Protein, ORF1a polyprotein;ORF1ab polyprotein Protein, ORF1ab Protein
Classe de substances
Viral Protein
Sujet
The viral nonstructural protein 1 (nsP1) is the only membrane-associated protein that anchors the replication complex to the cellular membranes. NSP1 inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.