Cette SARS-CoV-2 Spike protéine est marqué à la His tag.
Application
ELISA, SDS-PAGE (SDS)
Fonction
SARS-CoV-2 Spike Trimer, His Tag (B.1.1.529/Omicron) (MALS verified)
Attributs du produit
SARS-CoV-2 Spike Trimer, His Tag (B.1.1.529/Omicron) is the ectodomain of SARS-CoV-2 spike protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F). The 35 spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: B.1.1.529; GISAID clade: GR/484A; Nextstrain clade: 21K). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. Predicted N-terminus: Val 16
Pureté
> 95% as determined by SDS-PAGE. > 95% as determined by SEC-MALS.
Origine: SARS Coronavirus-2 (SARS-CoV-2)
Hôte: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Restrictions
For Research Use only
Format
Lyophilized
Buffer
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Conseil sur la manipulation
Please avoid repeated freeze-thaw cycles.
Stock
-20 °C/-80 °C
Stockage commentaire
For long term storage, the product should be stored at lyophilized state at -20°C or lower.This product is stable after storage at: 4-8°C for 12 months in lyophilized state, -70°C for 3 months under sterile conditions after reconstitution.
E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
Classe de substances
Viral Protein
Sujet
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.