SARS-CoV-2 Spike Protein (B.1.1.529 - Omicron, Trimer) (His-Avi Tag,Biotin)
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- Antigène Voir toutes SARS-CoV-2 Spike Protéines
- SARS-CoV-2 Spike
- Type de proteíne
- Recombinant
- Attributs du protein
- B.1.1.529 - Omicron, Trimer
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Origine
- SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Omicron
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette SARS-CoV-2 Spike protéine est marqué à la His-Avi Tag,Biotin.
- Application
- ELISA, SDS-PAGE (SDS)
- Fonction
- Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag™ (B.1.1.529/Omicron) (MALS verified)
- Specificité
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Attributs du produit
- Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (B.1.1.529/Omicron) is the ectodomain of SARS-CoV-2 spike protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F). The 35 spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: B.1.1.529; GISAID clade: GR/484A; Nextstrain clade: 21K). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. Predicted N-terminus: Val 16
- Pureté
- > 95% as determined by SDS-PAGE. > 95% as determined by SEC-MALS.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1.0 EU per μg by the LAL method.
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- Discover our top product SARS-CoV-2 Spike Protéine
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- Commentaires
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
- Conseil sur la manipulation
- Please avoid repeated freeze-thaw cycles.
- Stock
- -20 °C/-80 °C
- Stockage commentaire
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For long term storage, the product should be stored at lyophilized state at -20°C or lower.This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 months under sterile conditions after reconstitution. - Date de péremption
- 12 monts
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- Antigène
- SARS-CoV-2 Spike
- Abstract
- SARS-CoV-2 Spike Produits
- Synonymes
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- Classe de substances
- Viral Protein
- Sujet
- It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- Poids moléculaire
- 139.8 kDa
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