SARS-CoV-2 Spike Protein (B.1.621 - mu, RBD) (His-Avi Tag,Biotin)
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- Antigène Voir toutes SARS-CoV-2 Spike Protéines
- SARS-CoV-2 Spike
- Type de proteíne
- Recombinant
- Attributs du protein
- B.1.621 - mu, RBD
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Origine
- SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Mu
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette SARS-CoV-2 Spike protéine est marqué à la His-Avi Tag,Biotin.
- Fonction
- Biotinylated SARS-CoV-2 Spike RBD (R346K, E484K, N501Y), His,Avitag™ (MALS verified)
- Séquence
- Arg 319 - Lys 537
- Specificité
- SARS-CoV-2 Spike RBD (R346K, E484K, N501Y)
- Attributs du produit
- Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (SPD-C82Em) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (R346K, E484K, N501Y). The mutations R346K, E484K, N501Y were identified in the SARS-CoV-2 Mu variant (Pango lineage: B.1.621).
- Pureté
- >95 % as determined by SDS-PAGE.
- niveau d'endotoxine
- Less than 1.0 EU per μg by the LAL method.
- Classe de qualité
- MALS verified
- Top Product
- Discover our top product SARS-CoV-2 Spike Protéine
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- Indications d'application
- This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 28.2 kDa. The protein migrates as 34-38 kDa under reducing (R) condition due to glycosylation.
- Commentaires
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Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS, pH 7.4
- Stock
- -20 °C
- Stockage commentaire
- -20°C
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- Antigène
- SARS-CoV-2 Spike
- Autre désignation
- SARS-CoV-2 Spike (SARS-CoV-2 Spike Produits)
- Synonymes
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- Sujet
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Synonyms: Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD,
Description: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. - Poids moléculaire
- 28.2 kDa
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