SIGLEC7 Protein (His-Avi Tag,Biotin)
-
- Antigène Voir toutes SIGLEC7 Protéines
- SIGLEC7 (Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7))
- Type de proteíne
- Recombinant
- Origine
- Humain
-
Source
- HEK-293 Cells
- Purification/Conjugué
- Cette SIGLEC7 protéine est marqué à la His-Avi Tag,Biotin.
- Fonction
- Biotinylated Human Siglec-7 / CD328 Protein, His,Avitag™ (MALS & SPR verified)
- Séquence
- Gln 19 - Leu 353
- Attributs du produit
- Biotinylated Human Siglec-7, His,Avitag (SG7-H82E7) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Leu 353 (Accession # Q9Y286-1).
- Pureté
- >95 % as determined by SDS-PAGE.
- niveau d'endotoxine
- Less than 1.0 EU per μg by the LAL method.
- Classe de qualité
- MALS verified
- Top Product
- Discover our top product SIGLEC7 Protéine
-
-
- Indications d'application
- This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 40.5 kDa. The protein migrates as 55-65 kDa under reducing (R) condition due to glycosylation.
- Commentaires
-
Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Buffer
- 25 mM MES, 150 mM NaCl, pH 5.5
- Stock
- -20 °C
- Stockage commentaire
- -20°C
-
- Antigène
- SIGLEC7 (Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7))
- Autre désignation
- Siglec-7 / CD328 (SIGLEC7 Produits)
- Synonymes
- SIGLEC7 Protein, AIRM1 Protein, CD328 Protein, CDw328 Protein, D-siglec Protein, QA79 Protein, SIGLEC-7 Protein, SIGLEC19P Protein, SIGLECP2 Protein, p75 Protein, p75/AIRM1 Protein, sialic acid-binding Ig-like lectin 7 Protein, sialic acid binding Ig like lectin 7 Protein, LOC468976 Protein, SIGLEC7 Protein
- Sujet
-
Synonyms: CDw328,D-siglec,A79 membrane protein,p75,Adhesion inhibitory receptor molecule 1, AIRM-1,
Description: Siglec-7 is a member of the human CD33-related Siglec receptor. The extracellular region of Siglec-7 is characterized by an N-terminal V-set Ig domain that can bind sialic acid and two C2-set Ig domains. The cytoplasmic tail of Siglec-7 has one immune-receptor tyrosine-based inhibitory motif (ITIM) and one ITIM-like motif. Siglec-7 is considered as a sialic acid-dependent immunoreceptor with inhibitory potential and expressed predominantly on human NK cells, monocytes and a small subset of CD8+ T cells. - Poids moléculaire
- 40.5 kDa
- NCBI Accession
- NP_055200
-