PLD3 Protein (full length) (rho-1D4 tag)

N° du produit ABIN7126031

Détail du produit

Antigène: PLD3 (Phospholipase D family member 3 (PLD3))

Type de proteíne: Recombinant

Attributs du protein: full length

Origine: CHO cells

Source: Cellules d'insectes

Purification/Conjugué: Cette PLD3 protéine est marqué à la rho-1D4 tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys), Functional Studies (Func)

Séquence: MKPKLMYQEL KVPVEEPAGE LPVNEIEAWK AAEKKARWVL LVLILAVVGF GALMTQLFLW EYGDLHLFGP NQRPAPCYDP CEAVLVESIP EGLEFPNATT SNPSTSQAWL GLLAGAHSSL DIASFYWTLT NNDTHTQEPS AQQGEEILQQ LQALAPRGVK VRIAVSKPNG PLADLQSLLQ SGAQVRMVDM QKLTHGVLHT KFWVVDQTHF YLGSANMDWR SLTQVKELGV VMYNCSCLAR DLTKIFEAYW FLGQAGSSIP STWPRPFDTR YNQETPMEIC LNGTPALAYL ASAPPPLCPS GRTPDLKALL SVVDSARSFI YIAVMNYLPT MEFSHPRRFW PAIDDGLRRA AYERGVKVRL LVSCWGHSEP SMRSFLLSLA ALRDNHTHSD IQVKLFVVPA DEAQARIPYA RVNHNKYMVT ERAVYIGTSN WSGSYFTETA GTSLLVTQNG HDGLRSQLED VFLRDWNSLY SHNLDTAADS VGNACRLL
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO Phospholipase D family member 3 (Phospholipase D3) (Phospholipase D3-like protein) Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Classe de qualité: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PLD3 (Phospholipase D family member 3 (PLD3))

Autre désignation: Phospholipase D family member 3 (Phospholipase D3) (Phospholipase D3-like protein) (PLD3 Produits)

Synonymes: DDBDRAFT_0204850 Protein, DDBDRAFT_0231505 Protein, DDB_0204850 Protein, DDB_0231505 Protein, DDBDRAFT_0187542 Protein, DDBDRAFT_0220113 Protein, DDB_0187542 Protein, DDB_0220113 Protein, HU-K4 Protein, HUK4 Protein, Sam-9 Protein, phospholipase D3 Protein, phospholipase D family member 3 Protein, phospholipase D family, member 3 Protein, CpipJ_CPIJ017227 Protein, pldZ Protein, pldY Protein, DICPUDRAFT_30308 Protein, PLD3 Protein, Pld3 Protein

UniProt: G3HNQ5

Pathways: SARS-CoV-2 Protein Interactome