Aconitase 1 Protein (ACO1) (His tag)

N° du produit ABIN7194099

Détail du produit

Antigène: Aconitase 1 (ACO1)

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette Aconitase 1 protéine est marqué à la His tag.

Fonction: Recombinant Human ACO1/irp1 Protein (His Tag)

Séquence: Met 1-Lys 889

Attributs du produit: A DNA sequence encoding the human ACO1 (P21399) (Met 1-Lys 889) was expressed, with a polyhistidine tag at the N-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 10 % glycerol, 2 mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: Aconitase 1 (ACO1)

Autre désignation: ACO1/irp1 (ACO1 Produits)

Synonymes: ACONS Protein, IREB1 Protein, IREBP Protein, IREBP1 Protein, IRP1 Protein, Acon1 Protein, Ratireb Protein, ratireb Protein, Aco1 Protein, AI256519 Protein, Aco-1 Protein, Irebp Protein, Irp1 Protein, aconitase 1 Protein, aconitase 1, soluble Protein, aconitase 1 L homeolog Protein, ACO1 Protein, Aco1 Protein, aco1.L Protein

Sujet:

Background: Aconitase 1(ACO1) or IRP1 is one member of the aconitase family that contains a diverse group of iron-sulphur(Fe-S) isomerases and two types of iron regulatory protein. Aconitase exits in two forms: one is soluble and the other is mitochondrial. ACO1 is the soluble existing form, and the mitochondrial form is ACO2. Residues from all three N-terminal domains and the larger C-terminal domain contribute to the active site region. When the enzyme is activated, it gains an additional iron atom. ACO1 can assume two different functions in cells, depending on different conditions. During iron scarcity or oxidative stress, ACO1 binds to mRNA stem-loop structures called iron responsive elements to modulate the translation of iron metabolism genes. In iron-rich conditions, ACO1 binds an iron-sulfur cluster to function as a cytosolic aconitase. 

Synonym: ACONS;HEL60;IREB1;IREBP;IREBP1;IRP1

Poids moléculaire: 101 kDa

UniProt: P21399

Pathways: Transition Metal Ion Homeostasis