Aminoacylase 1 Protein (ACY1) (His tag)

N° du produit ABIN7317832

Détail du produit

Antigène: Aminoacylase 1 (ACY1)

Type de proteíne: Recombinant

Origine: Humain

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette Aminoacylase 1 protéine est marqué à la His tag.

Fonction: Recombinant Human ACY1/Aminoacylase-1 Protein (His Tag)

Séquence: Met 1-Ser 408

Attributs du produit: A DNA sequence encoding the full length of human ACY1 (NP_000657.1) (Met 1-Ser 408) was expressed with a polyhistidine tag at the C-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: Aminoacylase 1 (ACY1)

Autre désignation: ACY1/Aminoacylase-1 (ACY1 Produits)

Synonymes: si:zfos-226g1.1 Protein, zgc:55605 Protein, zgc:76955 Protein, acy1 Protein, acy1d Protein, acylase Protein, ACY1 Protein, ACY-1 Protein, ACY1D Protein, 1110014J22Rik Protein, Acy-1 Protein, Acy1a Protein, Acy1b Protein, aminoacylase 1 Protein, aminoacylase 1, gene 1 S homeolog Protein, aminoacylase 1, gene 1 Protein, ACY1 Protein, acy1 Protein, acy1.1.S Protein, acy1.1 Protein, Acy1 Protein

Sujet:

Background: Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine.

Synonym: ACY-1,ACY1D,HEL-S-5

Poids moléculaire: 47.3 kDa

NCBI Accession: NP_000657