BACE1 Protein (His tag)

N° du produit ABIN7318054

Détail du produit

Antigène: BACE1 (Beta-secretase 1 (BACE1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette BACE1 protéine est marqué à la His tag.

Fonction: Recombinant Human BACE1/ASP2 Protein (His Tag)(Active)

Séquence: Met 1-Thr 457

Attributs du produit: A DNA sequence encoding the extracellular domain (amino acid residue Met 1-Thr 457) of human BACE1 (NP_036236.1) precursor was expressed with a C-terminal polyhistidine tag.

Pureté: > 95 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2, R&D Systems, Catalog # ES004. The specific activity is >1.5 pmoles/min/μg.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: BACE1 (Beta-secretase 1 (BACE1))

Autre désignation: BACE1 (BACE1 Produits)

Synonymes: ASP2 Protein, BACE Protein, HSPC104 Protein, C76936 Protein, Bace Protein, MGC145931 Protein, BACE1 Protein, zgc:77409 Protein, beta-secretase 1 Protein, beta-site APP cleaving enzyme 1 Protein, beta-site APP-cleaving enzyme 1 Protein, BACE1 Protein, Bace1 Protein, bace1 Protein

Sujet:

Background: Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.

Synonym: ASP2,BACE,HSPC104

Poids moléculaire: 49.9 kDa

NCBI Accession: NP_036236