Carboxypeptidase A2 Protein (His tag)

N° du produit ABIN7194599

Détail du produit

Antigène: Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette Carboxypeptidase A2 protéine est marqué à la His tag.

Fonction: Recombinant Human Carboxypeptidase A2/CPA2 Protein (His Tag)(Active)

Séquence: Met 1-Tyr 417

Attributs du produit: A DNA sequence encoding the pre pro form of human CPA2 (NP_001860.2) (Met 1-Tyr 417) was expressed with a C-terminal polyhistidine tag.

Pureté: > 90 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave a colorimetric peptide substrate, N-acetyl-Phe-Thiaphe-OH (N-Ac-PSP, Peptide International's Catalog# STP-3621-PI), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), as measured using the wavelength at 405 nm and the extinction coefficient of 13,260 M-1 cm-The specific activity is >4,000 pmoles/min/μg.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 25 mM Tris, 0.15 mM NaCl, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))

Autre désignation: Carboxypeptidase A2/CPA2 (CPA2 Produits)

Synonymes: CPA2 Protein, zgc:92530 Protein, carboxypeptidase A2 Protein, carboxypeptidase A2 (pancreatic) Protein, Carboxypeptidase A2 Protein, carboxypeptidase A2, pancreatic Protein, CPA2 Protein, cpa2 Protein, CpipJ_CPIJ001743 Protein, cbpa2 Protein, Cpa2 Protein

Sujet:

Background: Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.

Synonym: Carboxypeptidase A2, CPA2

Poids moléculaire: 46 kDa

NCBI Accession: NP_001860