CSF1R Protein (His tag)
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- Antigène Voir toutes CSF1R Protéines
- CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
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Origine
- Souris
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette CSF1R protéine est marqué à la His tag.
- Fonction
- Recombinant Mouse CSF1R/CD115 Protein (His Tag)(Active)
- Séquence
- Met 1-Ser 511
- Attributs du produit
- A DNA sequence encoding the mouse CSF1R (NP_001032948.2) extracellular domain (Met 1-Ser 511) was fused with a polyhistidine tag at the C-terminus.
- Pureté
- > 97 % as determined by SDS-PAGE
- niveau d'endotoxine
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- 1. Measured by its ability to bind recombinant mouse IL34 in a functional ELISA.2. Measured by its binding ability in a functional ELISA.3. Immobilized mouse CSF1R-His at 10 μg/mL (100 μl/well) can bind biotinylated human CSF1-his, The EC50 of biotinylated human CSF1-his is 0.50 μg/mL.
- Top Product
- Discover our top product CSF1R Protéine
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Antigène
- CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))
- Autre désignation
- CSF1R/CD115 (CSF1R Produits)
- Synonymes
- AI323359 Protein, CD115 Protein, CSF-1R Protein, Csfmr Protein, Fim-2 Protein, Fms Protein, M-CSF-R Protein, M-CSFR Protein, C-FMS Protein, CSFR Protein, FIM2 Protein, FMS Protein, HDLS Protein, PDGFRB Protein, MGC89146 Protein, CSF-1-R Protein, c-fms Protein, mrfms Protein, CSF1 Protein, M-CSF Protein, csf1r Protein, fms Protein, panther Protein, colony stimulating factor 1 receptor Protein, macrophage colony-stimulating factor receptor Protein, colony stimulating factor 1 receptor, a Protein, Csf1r Protein, CSF1R Protein, m-csfr Protein, csf1r Protein, csf1ra Protein
- Sujet
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Background: M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation, differentiation, and activation of macrophages. This cell surface glycoprotein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. Binding of CSF1 activates the receptor kinase, leading to "autophosphorylation" of receptor subunits and the concomitant phosphorylation of a series of cellular proteins on tyrosine residues. CSF1R is a tyrosine kinase receptor that is absolutely required for macrophage differentiation and thus occupies a central role in hematopoiesis. CSF1 and its receptor (CSF1R, product of c-fms proto-oncogene) were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role for CSF1/CSF1R in normal mammary gland development is very intriguing because this receptor/ligand pair has also been found to be important in the biology of breast cancer in which abnormal expression of CSF1 and its receptor correlates with tumor cell invasiveness and adverse clinical prognosis. Tumor cell expression of CSF1R is under the control of several steroid hormones (glucocorticoids and progestins) and the binding of several bHLH transcription factors, while tumor cell expression of CSF-1 appears to be regulated by other hormones, some of which are involved in normal lactogenic differentiation. However, studies have demonstrated that CSF1 and CSF1R have additional roles in mammary gland development during pregnancy and lactation. The role of CSF1 and CSF1R in normal and neoplastic mammary development that may elucidate potential relationships of growth factor-induced biological changes in the breast during pregnancy and tumor progression.
Synonym: AI323359;CD115;CSF-1R;Csfmr;Fim-2;Fms;M-CSF-R;M-CSFR
- Poids moléculaire
- 56.8 kDa
- NCBI Accession
- NP_001032948
- Pathways
- Signalisation RTK, Inositol Metabolic Process, Cell-Cell Junction Organization
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