GFRA1 Protein (AA 1-424) (His tag)
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- Antigène Voir toutes GFRA1 Protéines
- GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- AA 1-424
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette GFRA1 protéine est marqué à la His tag.
- Fonction
- Recombinant Human GFRA1/GDNFRA Protein (aa 1-424, His Tag)(Active)
- Séquence
- Met 1-Ser 424
- Attributs du produit
- A DNA sequence encoding the human GFRα1 without the propeptide (NP_665736.1) (Met 1-Ser 424) was fused with the a polyhistidine tag at the C-terminus.
- Pureté
- > 98 % as determined by reducing SDS-PAGE.
- niveau d'endotoxine
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- 1. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.2-1 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF.2. Measured by its ability to bind human GDNF (native) in functional ELISA.
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- Discover our top product GFRA1 Protéine
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Antigène
- GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))
- Autre désignation
- GFRA1/GDNFRA (GFRA1 Produits)
- Synonymes
- gdnfr Protein, ret1l Protein, retl1 Protein, trnr1 Protein, gdnfra Protein, MGC122616 Protein, gfr-alpha-1 Protein, GDNFR Protein, GDNFRA Protein, GFR-ALPHA-1 Protein, RET1L Protein, RETL1 Protein, TRNR1 Protein, GDNFRALPHA Protein, GFRA1 Protein, AU042498 Protein, gfralpha1a Protein, zgc:110129 Protein, GDNF family receptor alpha 1 Protein, glial cell line derived neurotrophic factor family receptor alpha 1 Protein, gdnf family receptor alpha 1a Protein, gfra1 Protein, GFRA1 Protein, Gfra1 Protein, gfra1a Protein
- Sujet
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Background: Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 1 (GFRA1) is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA1 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. GDNF, a distantly related member of the transforming growth factor-β (TGF-â) superfamily, and its receptor components: GFRA1, Ret and neural cell adhesion molecule (NCAM) have been recently reported to be expressed in the testis and to be involved in the proliferation regulation of immature Sertoli cells.
Synonym: GDNF Family Receptor Alpha-1; GDNF Receptor Alpha-1; GDNFR-Alpha-1; GFR-Alpha-1; RET Ligand 1; TGF-Beta-Related Neurotrophic Factor Receptor 1; GFRA1; GDNFRA; RETL1; TRNR1
- Poids moléculaire
- 46 kDa
- NCBI Accession
- NP_665736
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