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LGMN Protein (His tag)

LGMN Origine: Souris Hôte: HEK-293 Cells Recombinant > 75 % as determined by SDS-PAGE Active
N° du produit ABIN7196760
  • Antigène Voir toutes LGMN Protéines
    LGMN (Legumain (LGMN))
    Type de proteíne
    Recombinant
    Activité biologique
    Active
    Origine
    • 7
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Source
    • 8
    • 4
    • 2
    • 2
    • 1
    HEK-293 Cells
    Purification/Conjugué
    Cette LGMN protéine est marqué à la His tag.
    Fonction
    Recombinant Mouse Legumain/LGMN Protein (His Tag)(Active)
    Séquence
    Val 18-Tyr 435
    Attributs du produit
    A DNA sequence encoding the extracellular domain (Val 18-Tyr 435) of mouse LGMN (NP_035305.1) precursor was expressed with a C-terminal polyhistidine tag.
    Pureté
    > 75 % as determined by SDS-PAGE
    niveau d'endotoxine
    < 1.0 EU per μg of the protein as determined by the LAL method.
    Biological Activity Comment
    Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methyl coumarin(Z-AAN-AMC).The specific activity is > 350 pmoles/min/μg.(Activation description: The enzyme achieves its activity under acidic pH)
    Top Product
    Discover our top product LGMN Protéine
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Please refer to the printed manual for detailed information.
    Buffer
    Lyophilized from sterile 25 mM Tris, 0.15M NaCl, 20 % Glycerol, pH 7.5
    Stock
    4 °C,-20 °C,-80 °C
    Stockage commentaire
    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Antigène
    LGMN (Legumain (LGMN))
    Autre désignation
    Legumain/LGMN (LGMN Produits)
    Synonymes
    AEP Protein, LGMN1 Protein, PRSC1 Protein, AI746452 Protein, AU022324 Protein, Prsc1 Protein, MGC64351 Protein, cb173 Protein, cb460 Protein, sb:cb173 Protein, zgc:76953 Protein, wu:fa98g05 Protein, wu:fj92g08 Protein, lgmn Protein, MGC88980 Protein, DELTA VACUOLAR PROCESSING ENZYME Protein, DELTAVPE Protein, delta vacuolar processing enzyme Protein, legumain Protein, legumain L homeolog Protein, delta vacuolar processing enzyme Protein, Legumain Protein, LGMN Protein, Lgmn Protein, lgmn.L Protein, lgmn Protein, DELTA-VPE Protein, MBOO_RS07785 Protein, Odosp_3582 Protein, LOC569153 Protein
    Sujet

    Background: The Mammalian Legumain, also known as LGMN, also called asparaginyl endopeptidase (AEP), is a cysteine protease belonging to peptidase family C13 with a strict specificity for hydrolysis of asparaginyl bonds. Known previously only from plants and invertebrates, Legumain is discovered as a lysosomal endopeptidase in mammals. Mammalian Legumain is a cysteine endopeptidase, inhibited by iodoacetamide and maleimides, but unaffected by compound E64. The Mammalian Legumain is involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Legumain has been observed to be highly expressed in several types of solid tumors. It was demonstrated in membrane-associated vesicles concentrated at the invadopodia of tumor cells and on cell surfaces where it colocalized with integrins. Legumain was demonstrated to activate progelatinase A. Cells overexpressing Legumain possessed increased migratory and invasive activity in vitro and adopted an invasive and metastatic phenotype in vivo, inferring significance of Legumain in tumor invasion and metastasis. In addition, Legumain is expressed in both murine and human atherosclerotic lesions. The macrophage-specific expression of Legumain in vivo and ability of Legumain to induce chemotaxis of monocytes and endothelial cells in vitro suggest that Legumain may play a functional role in atherogenesis.

    Synonym: Legumain,Lgmn,Asparaginyl endopeptidase,Protease cysteine 1,Prsc1,AEP

    Poids moléculaire
    49.8 kDa
    NCBI Accession
    NP_035305
    Pathways
    Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades
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