KARS Protein (His tag)

N° du produit ABIN7318750

Détail du produit

Antigène: KARS (Lysyl-tRNA Synthetase (KARS))

Type de proteíne: Recombinant

Origine: Humain

Source: Human Cells

Purification/Conjugué: Cette KARS protéine est marqué à la His tag.

Fonction: Recombinant Human KARS Protein (His Tag)

Séquence: Ala2-Val597

Attributs du produit: Recombinant Human Lysine--tRNA Ligase is produced by our Mammalian expression system and the target gene encoding Ala2-Val597 is expressed with a 6His tag at the C-terminus.

Pureté: > 95 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,1 mM DTT,20 % glycerol, pH 8.0.

Stock: -20 °C

Stockage commentaire: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Détail du antigène

Antigène: KARS (Lysyl-tRNA Synthetase (KARS))

Autre désignation: KARS (KARS Produits)

Synonymes: CG12141 Protein, Dmel\\CG12141 Protein, KRS Protein, LysRS Protein, cb530 Protein, wu:fa16h02 Protein, zgc:92483 Protein, kars Protein, MGC53345 Protein, kars2 Protein, krs Protein, krs-1 Protein, KARS Protein, Tb06.28P18.190 Protein, CMTRIB Protein, DFNB89 Protein, KARS1 Protein, KARS2 Protein, AA589550 Protein, AL024334 Protein, AL033315 Protein, AL033367 Protein, D8Ertd698e Protein, D8Wsu108e Protein, mKIAA0070 Protein, Lysyl-tRNA synthetase Protein, lysyl-tRNA synthetase Protein, lysyl-tRNA synthetase S homeolog Protein, lysyl-tRNA synthetase L homeolog Protein, LysRS Protein, kars Protein, kars.S Protein, kars.L Protein, Bm1_16500 Protein, lysS Protein, Taci_1118 Protein, Tpau_1737 Protein, Arch_0261 Protein, Trad_2960 Protein, Spirs_3699 Protein, KARS Protein, LOC100280510 Protein, Tb927.6.1510 Protein, Kars Protein

Sujet:

Background: Lysine-tRNA ligase, also known as Lysyl-tRNA synthetase, LysRS, KARS and KIAA0070, belongs to the class-II aminoacyl-tRNA synthetase family. The N-terminal cytoplasmic domain (1-65) is a functional tRNA-binding domain, which is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. KARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB).

Synonym: Lysine--tRNA Ligase, Lysyl-tRNA Synthetase, LysRS, KARS, KIAA0070

Poids moléculaire: 69.1 kDa

UniProt: Q15046